sechenovSechenov Medical JournalСеченовский вестник2218-73322658-3348Сеченовский Университет10.47093/2218-7332.2020.11.3.47-56sechenov-222Research ArticlePATHOLOGICAL PHYSIOLOGYПАТОЛОГИЧЕСКАЯ ФИЗИОЛОГИЯVEGF-C — a biomarker of renal injury in the experimental model of intra-abdominal hypertensionVEGF-C — биомаркер повреждения почек при экспериментальной интраабдоминальной гипертензииhttps://orcid.org/0000-0002-1833-3028ЯковлевВ. В.IakovlevV. V.

Яковлев Владислав Вячеславович, аспирант кафедры патофизиологии

ул. Трубецкая, д. 8, стр. 2, г. Москва, 119991

+7 (926) 761-20-68

Vladislav V. Iakovlev, Postgraduate, Pathophysiology Department

8/2, Trubetskaya str., Moscow, 119991

8 (926) 761-20-68

vladislav.iak@gmail.comhttps://orcid.org/0000-0002-9870-4414БадаеваА. В.BadaevaA. V.

Бадаева Анастасия Викторовна, студентка

ул. Трубецкая, д. 8, стр. 2, г. Москва, 119991

Anastasiia V. Badaeva, student

8/2, Trubetskaya str., Moscow, 119991

nastyabadaeva1507@gmail.comhttps://orcid.org/0000-0002-5601-0465ИвановаЕ. И.IvanovaE. I.

Иванова Елена Ильинична, врач-патологоанатом

пер. Абрикосовский, д. 2, ГСП-1, г. Москва, 119991

Elena I. Ivanova, pathologist

2, Abrikosovsky lane, GSP-1, Moscow, 119991

ellen151@rambler.ruhttps://orcid.org/0000-0003-2422-3463СевергинаЛ. О.SeverginaL. O.

Севергина Любовь Олеговна, д-р мед. наук, врачпатологоанатом, профессор кафедры патологической анатомии им. А.И. Струкова

ул. Трубецкая, д. 8, стр. 2, г. Москва, 119991

Lyubov O. Severgina, Dr. of Sci. (Medicine), pathologist, Professor, Department of Pathological Anatomy named after A.I. Strukov

8/2, Trubetskaya str., Moscow, 119991

losevergina@gmail.comhttps://orcid.org/0000-0002-4380-4522МальцеваЛ. Д.MaltsevaL. D.

Мальцева Лариса Дмитриевна, канд. мед. наук, доцент кафедры патофизиологии

ул. Трубецкая, д. 8, стр. 2, г. Москва, 119991

Larisa D. Maltseva, Cand. of Sci. (Medicine), Associate Professor, Pathophysiology Department

8/2, Trubetskaya str., Moscow, 119991

lamapost@mail.ruhttps://orcid.org/0000-0003-2453-1319МорозоваО. Л.MorozovaO. L.

Морозова Ольга Леонидовна, д-р мед. наук, профессор кафедры патофизиологии

ул. Трубецкая, д. 8, стр. 2, г. Москва, 119991

Olga L. Morozova, Dr. of Sci. (Medicine), Professor, Pathophysiology Department

8/2, Trubetskaya str., Moscow, 119991

morozova_ol@list.ruФГАОУ ВО «Первый Московский государственный медицинский университет им. И.М. Сеченова» Минздрава России (Сеченовский Университет)РоссияSechenov First Moscow State Medical University (Sechenov University)Russian FederationФГБНУ «Российский научный центр хирургии им. академика Б.В. Петровского»РоссияPetrovsky National Research Centre of SurgeryRussian Federation2020251120201134756Copyright © Iakovlev V.V., Badaeva A.V., Ivanova E.I., Severgina L.O., Maltseva L.D., Morozova O.L., 20202020Яковлев В.В., Бадаева А.В., Иванова Е.И., Севергина Л.О., Мальцева Л.Д., Морозова О.Л.Iakovlev V.V., Badaeva A.V., Ivanova E.I., Severgina L.O., Maltseva L.D., Morozova O.L.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.sechenovmedj.com/jour/article/view/222

Lymphangiogenesis plays an important role in development of renal parenchyma inflammation during kidney injury. Vascular endothelial growth factor type C (VEGF-C), cytokine that regulates lymphangiogenesis, is a potential early biomarker for acute kidney injury. Aim. To study the concentration of VEGF-C in renal homogenate and blood serum of newborn rats with experimental intraabdominal hypertension (IAH) of varying severity and duration, to establish a relationship with morphological changes in the renal tissue. Materials and methods. The experiment was conducted on 50 newborn Wistar rats. Rats were divided into 5 groups of 10 rats each: groups 1 and 2 with mild IAH lasting 5 and 10 days, respectively, and groups 3 and 4 with severe IAH lasting 5 and 10 days, respectively, and the control group. IAH was modelled by injecting sterile vaseline into the abdominal cavity to a predetermined level of IAH under the control of intra-vesical manometry. VEGF-C content was measured by ELISA. Morphological examination of the biopsy material and its photography were carried out using a Leica DM2000 microscope. The Mann—Whitney, Kruskal—Wallis, Wilcoxon tests, as well as one-way ANOVA, were used for statistical analysis. Results. The level of VEGF-C in the renal homogenate was increased in all groups (pc < 0.001); the degree of VEGF-C increase depended on the severity of IAH (p < 0.05) but not on the duration of IAH exposure. The VEGF-C blood serum level was increased only in group 3 (pc = 0.011). Morphological analysis showed hydropic dystrophy: changes in the height of the tubular epithelium, an increase in interstitial edema, expansion of the urinary spaces of glomeruli. The degree of morphological changes depended on the severity and duration of IAH. Conclusion. Changes in VEGF-C level assessed in the renal homogenate correlated with morphological changes in renal tissue of rats with different severity and duration of IAH.

Лимфангиогенез играет важную роль в развитии воспаления паренхимы почек при их повреждении. Васкулоэндотелиальный фактор роста тип С (vascular endothelial growth factor, VEGF-C) — цитокин, регулирующий лимфангиогенез, — является потенциальным ранним биомаркером острого повреждения почек.Цель. Изучить содержание VEGF-C в почечном гомогенате и сыворотке крови новорожденных крыс с экспериментальной интраабдоминальной гипертензией (ИАГ) различной тяжести и длительности, установить взаимосвязь с морфологическими изменениями в паренхиме почек.Материалы и методы. Эксперимент проведен на 50 новорожденных крысах линии Wistar, которые были разделены на 5 групп по 10 особей: группы 1 и 2 с легкой ИАГ длительностью 5 и 10 дней соответственно, группы 3 и 4 с тяжелой ИАГ длительностью 5 и 10 дней соответственно и 5-я контрольная группа. ИАГ достигалась введением стерильного вазелина в брюшную полость до заданного уровня интраабдоминального давления под контролем интравезикальной манометрии. Содержание VEGF-C измерялось методом ELISA. Морфологическое исследование биопсийного материала и его фотосъемка осуществлялись на микроскопе Leica DM2000. Сравнение групп проводилось с помощью критериев Манна — Уитни, Краскела — Уоллиса, Вилкоксона; проводился однофакторный дисперсионный анализ.Результаты. Отмечено увеличение концентрации VEGF-C в почечном гомогенате во всех экспериментальных группах (pк < 0,001). Степень повышения VEGF-C зависела от тяжести ИАГ (p < 0,05), но не от длительности экспозиции ИАГ. Содержание VEGF-C в сыворотке крови было повышено только в 3-й группе (pк = 0,011). При морфологическом исследовании установлены изменения, которые характерны для гидропической дистрофии: изменение высоты канальцевого эпителия, нарастание отека интерстиция, расширение мочевых пространств гломерул. Степень морфологических изменений паренхимы почек зависела от тяжести и длительности ИАГ.Заключение. Наблюдалась взаимосвязь между морфологическими изменениями паренхимы почек при ИАГ различной тяжести и длительности и изменением уровня VEGF-C в почечном гомогенате.

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The authors declare that there are no conflicts of interest present.