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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="en"><front><journal-meta><journal-id journal-id-type="publisher-id">sechenov</journal-id><journal-title-group><journal-title xml:lang="en">Sechenov Medical Journal</journal-title><trans-title-group xml:lang="ru"><trans-title>Сеченовский вестник</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2218-7332</issn><issn pub-type="epub">2658-3348</issn><publisher><publisher-name>Сеченовский Университет</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.47093/2218-7332.2021.306.04</article-id><article-id custom-type="elpub" pub-id-type="custom">sechenov-350</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>BIOMEDICINE</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>БИОМЕДИЦИНА</subject></subj-group></article-categories><title-group><article-title>Study of the physical and biological properties of nanocomposite materials obtained with laser radiation</article-title><trans-title-group xml:lang="ru"><trans-title>Исследование физических и биологических свойств нанокомпозитных материалов, полученных с использованием лазерного излучения</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-3388-5146</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Курилова</surname><given-names>У. Е.</given-names></name><name name-style="western" xml:lang="en"><surname>Kurilova</surname><given-names>U. E.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Курилова Ульяна Евгеньевна - инженер Института биомедицинских систем. </p><p>Пл. Шокина, д. 1, Зеленоград,  Москва, 124498, Тел.: +7 (917) 583-10-90</p></bio><bio xml:lang="en"><p>Uliana E. Kurilova - engineer, Institute of Biomedical Systems.</p><p>1, Shokin Square, Zelenograd, Moscow, 124498, Russia Tel.: +7 (917) 583-10-90</p></bio><email xlink:type="simple">kurilova_10@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-6514-2411</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Герасименко</surname><given-names>А. Ю.</given-names></name><name name-style="western" xml:lang="en"><surname>Gerasimenko</surname><given-names>A. Yu.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Герасименко Александр Юрьевич - кандидат физико-математических наук, доцент, старший научный сотрудник.</p><p>Пл. Шокина, д. 1, Зеленоград, Москва, 124498</p></bio><bio xml:lang="en"><p>Alexander Yu. Gerasimenko - Cand. of Sci. (Physics and Mathematics), Associate Professor, Senior Researcher, Institute of Biomedical Systems.</p><p> 1, Shokin Square, Zelenograd, Moscow, 124498</p></bio><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>ФГАОУ ВО Национальный исследовательский университет Московский институт электронной техники</institution><country>Россия</country></aff><aff xml:lang="en"><institution>National Research University of Electronic Technology</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2021</year></pub-date><pub-date pub-type="epub"><day>14</day><month>12</month><year>2021</year></pub-date><volume>12</volume><issue>3</issue><fpage>31</fpage><lpage>37</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Kurilova U.E., Gerasimenko A.Y., 2021</copyright-statement><copyright-year>2021</copyright-year><copyright-holder xml:lang="ru">Курилова У.Е., Герасименко А.Ю.</copyright-holder><copyright-holder xml:lang="en">Kurilova U.E., Gerasimenko A.Y.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.sechenovmedj.com/jour/article/view/350">https://www.sechenovmedj.com/jour/article/view/350</self-uri><abstract><p>The new method of the formation of nanocomposite materials based on carbon nanotubes for the regeneration of connective tissues has been developed.</p><sec><title>Aim</title><p>Aim. Study of the structure, mechanical characteristics and biocompatibility of the obtained materials.</p></sec><sec><title>Materials and methods</title><p>Materials and methods. The experimental samples of nanocomposite materials were based on multi-walled and singlewalled carbon nanotubes, the matrix was bovine serum albumin. A layer of liquid dispersion of the components on a silicon substrate or in a container was irradiated with laser radiation to form the solid nanocomposite material. The microstructure of the obtained samples was analyzed with X-ray microtomography, the tensile strength was investigated using a testing machine. Fibroblast cells were incubated with experimental samples for 3, 24, 48, and 72 h and then fixed with glutaraldehyde. Cell growth during incubation with samples was studied using optical and atomic force microscopy.</p></sec><sec><title>Results</title><p>Results. It was found that a slight decrease in tensile strength and increase in the degree of deformation were observed with an increase in the concentration of carbon nanotubes. At the same time, the mechanical parameters of the samples corresponded to the requirements for materials for the restoration of connective tissue defects. Microscopic studies indicate good adhesion of cells to the nanocomposite material, no toxic effect of the samples on the cells was found. After 3 hours of incubation, the cells had their original rounded shape, after 24 hours of incubation cells began to proliferate on the sample's surface and were spindle-shaped. After 48 and 72 hours, the cells practically formed a monolayer on the surface of the samples.</p></sec><sec><title>Conclusion</title><p>Conclusion. The results of the study show that the structural and mechanical parameters of the developed nanocomposite materials meet the requirements of biomedicine. It was also shown that nanocomposite materials do not suppress cell growth and can serve as a scaffold for the regeneration of damaged tissues.</p></sec></abstract><trans-abstract xml:lang="ru"><p>Разработан новый метод формирования нанокомпозитных материалов на основе углеродных нанотрубок для регенерации соединительных тканей организма.</p><sec><title>Цель</title><p>Цель. Исследование структуры, механических характеристик и биосовместимости полученных материалов.</p></sec><sec><title>Материалы и методы</title><p>Материалы и методы. Основой экспериментальных образцов являлись многостенные и одностенные углеродные нанотрубки, в качестве матрицы использовался бычий сывороточный альбумин. Слой жидкой дисперсии компонентов на кремниевой подложке или в емкости обрабатывался лазерным излучением с образованием объемного нанокомпозитного материала в твердой фазе. Микроструктура полученных образцов была исследована методом рентгеновской микротомографии, прочность на разрыв исследовалась с помощью испытательной машины. Клетки фибробласты инкубировались с экспериментальными образцами в течение 3, 24, 48 и 72 часов, а затем фиксировались глутаровым альдегидом. Рост клеток во время инкубации с образцами был изучен с помощью оптической и атомно-силовой микроскопии.</p></sec><sec><title>Результаты</title><p>Результаты. Установлено, что с увеличением концентрации углеродных нанотрубок наблюдается небольшое снижение прочности и увеличение степени деформации. При этом механические параметры образцов соответствовали требованиям, предъявляемым к материалам для восстановления дефектов соединительных тканей. Микроскопические исследования указывают на высокую степень адгезии в процессе взаимодействия клеток с нанокомпозитным материалом, токсического действия образцов на клетки не было обнаружено. Через 3 часа инкубации клетки имели первоначальную округлую форму. Клетки на образцах после 24 часов инкубации начали распространяться по поверхности образцов и имели веретенообразную форму. Через 48 и 72 часа клетки практически образовывали монослой на поверхности образцов.</p></sec><sec><title>Заключение</title><p>Заключение. Результаты исследования показывают, что структурные и механические параметры разработанных нанокомпозитных материалов удовлетворяют требованиям биомедицины. Также было показано, что нанокомпозитные материалы не подавляют рост клеток и могут служить в качестве каркаса для регенерации поврежденных тканей.</p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>углеродные нанотрубки</kwd><kwd>сывороточный альбумин</kwd><kwd>каркас</kwd><kwd>лазерное структурирование</kwd><kwd>атомносиловая микроскопия</kwd><kwd>тканевая инженерия</kwd><kwd>фибробласты</kwd><kwd>дефекты соединительной ткани</kwd></kwd-group><kwd-group xml:lang="en"><kwd>carbon nanotubes</kwd><kwd>serum albumin</kwd><kwd>scaffold</kwd><kwd>laser structuring</kwd><kwd>atomic force microscopy</kwd><kwd>tissue engineering</kwd><kwd>fibroblasts</kwd><kwd>connective tissue defects</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Работа выполнена в рамках государственного задания Министерства науки и высшего образования Российской Федерации (соглашение № 075-03-2020-216 от 27.12.2019)</funding-statement><funding-statement xml:lang="en">The study was supported by the Ministry of Science and Higher Education of the Russian Federation (No. 075-032020-216 from 27.12.2019)</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Rider P., Kacarevic Z.P., Alkildani S., et al. 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