sechenovSechenov Medical JournalСеченовский вестник2218-73322658-3348Сеченовский Университетsechenov-805Research ArticlePHARMACEUTICAL CHEMISTRYФАРМАЦЕВТИЧЕСКАЯ ХИМИЯTHE STUDY OF INTESTINAL PERMEABILITY IN VITRO ON A MONOLAYER OF EPITHELIAL CACO-2 CELLS (REVIEW)ИЗУЧЕНИЕ КИШЕЧНОЙ ПРОНИЦАЕМОСТИ В УСЛОВИЯХ IN VITRO НА МОНОСЛОЕ ЭПИТЕЛИАЛЬНЫХ КЛЕТОК САСО-2 ( ОБЗОР )ШохинИ. Е.ShokhinI. E.

И.Е. Шохин, к.фарм.н., ассистент кафедры фармацевтической и токсикологической химии

I.E. Shokhin, PhD, assistant of the chair of pharmaceutical and toxicological chemistry

РаменскаяГ. В.RamenskayaG. V.

Галина Владиславовна Раменская, д.фарм.н., профессор, заведующая кафедрой фармацевтической и токсикологической химии, директор НИИ фармации8 (495) 691–13–92

119019, г. Москва, ул. Никитский бульвар, д. 13

G.V. Ramenskaya, Doctor of pharmacy, prof., head of the chair of pharmaceutical and toxicological chemistry, director of the Research institute of pharmacy

ramenskaia@mail.ruКулиничЮ. И.KulinichY. I.

Ю.И. Кулинич, аспирантка кафедры фармацевтической и токсикологической химии

Yu.I. Kulinich, post-graduate student of the chair of pharmaceutical and toxicological chemistry

СавченкоА. Ю.SavchenkoA. Y.

А.Ю. Савченко, к.м.н., заместитель директора по инновационному развитию НИИ фармации

A.Yu. Savchenko, PhD, deputy director of innovative development of the Research institute of pharmacy

Первый МГМУ им. И.М. СеченоваРоссияFirst MSMU named after I.M. SechenovRussian Federation201230092012033135Copyright © Shokhin I.E., Ramenskaya G.V., Kulinich Y.I., Savchenko A.Y., 20122012Шохин И.Е., Раменская Г.В., Кулинич Ю.И., Савченко А.Ю.Shokhin I.E., Ramenskaya G.V., Kulinich Y.I., Savchenko A.Y.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.sechenovmedj.com/jour/article/view/805

This paper is devoted to the application of epithelial monolayer of human colon adenocarcinoma cells — Caco-2 for in vitro permeability evaluation of drugs. Main advantages of this biological model are described. General principles, approaches and methodology of Caco-2 permeability assay are defined. Correlation between human intestinal permeability in vitro and apparent permeability coefficient in vitro is indicated

Статья посвящена применению монокультуры клеток аденокарциномы толстого кишечника Caco-2 для оценки проницаемости лекарственных веществ in vitro. Описаны основные преимущества использования данной биологической модели. Приведены общие принципы, подходы и методология определения проницаемости на культуре клеток Сасо-2. Показана корреляция между значениями кишечной проницаемости, определенной в условиях in vivo, и результатами in vitro.

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The authors declare that there are no conflicts of interest present.