sechenovSechenov Medical JournalСеченовский вестник2218-73322658-3348Сеченовский Университет10.47093/2218-7332.2023.14.3.28-36sechenov-971Research ArticleCELL BIOLOGY, CYTOLOGY, HISTOLOGYКЛЕТОЧНАЯ БИОЛОГИЯ, ЦИТОЛОГИЯ, ГИСТОЛОГИЯAge-dependent patterns of somatostatinergic neurons in sympathetic paravertebral gangliaВозрастное развитие соматостатинергических нейронов симпатических превертебральных узловhttps://orcid.org/0000-0003-3685-270XЕмануйловА. И.EmanuilovA. I.

Емануйлов Андрей Игоревич - канд. мед. наук, доцент кафедры анатомии человека

ул. Революционная, д. 5, г. Ярославль, 150000

Andrey I. Emanuilov - Cand. of Sci. (Medicine), Associate Professor, Human Anatomy Department 

5, Revolyutsionnaya str., Yaroslavl, 150000

https://orcid.org/0000-0003-2814-7656ПорсеваВ. В.PorsevaV. V.

Порсева Валентина Вячеславовна - д-р мед. наук, доцент кафедры патофизиологии 

ул. Революционная, д. 5, г. Ярославль, 150000

Valentina V. Porseva - Dr. of Sci. (Medicine), Associate Professor, Human Pathology Department

5, Revolyutsionnaya str., Yaroslavl, 150000

https://orcid.org/0000-0001-8227-9997ПавловА. В.PavlovA. V.

Павлов Алексей Владимирович - д-р мед. наук, профессор, заведующий кафедрой гистологии 

ул. Революционная, д. 5, г. Ярославль, 150000

Alexei V. Pavlov - Dr. of Sci. (Medicine), Professor, Head of the Histology Department

5, Revolyutsionnaya str., Yaroslavl, 150000

https://orcid.org/0000-0002-6230-5024МаслюковП. М.MasliukovP. M.

Маслюков Петр Михайлович - д-р мед. наук, профессор, заведующий кафедрой нормальной физиологии ул. Революционная, д. 5, г. Ярославль, 150000

Тел.: +7 (910) 972-88-61

Petr M. Masliukov - Dr. of Sci. (Medicine), Professor, Head of the Normal Physiology Department

5, Revolyutsionnaya str., Yaroslavl, 150000

Tel.: +7 (910) 972-88-61

mpm@ysmu.ruФГБОУ ВО «Ярославский государственный медицинский университет» Минздрава РоссииРоссияYaroslavl State Medical UnivesityRussian FederationФГБОУ ВО «Ярославский государственный медицинский университет» Минздрава РоссииРоссияYaroslavl State Medical UnivesityRussian FederationФГБОУ ВО «Ярославский государственный медицинский университет» Минздрава РоссииРоссияYaroslavl State Medical UnivesityRussian FederationФГБОУ ВО «Ярославский государственный медицинский университет» Минздрава РоссииРоссияYaroslavl State Medical UnivesityRussian Federation202320102023143Special issue «Cell biology, citology, histology»2836Copyright © Emanuilov A.I., Porseva V.V., Pavlov A.V., Masliukov P.M., 20232023Емануйлов А.И., Порсева В.В., Павлов А.В., Маслюков П.М.Emanuilov A.I., Porseva V.V., Pavlov A.V., Masliukov P.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.sechenovmedj.com/jour/article/view/971Aim

Aim. We aimed to determine the content of neurons expressing somatostatin (SST) and their colocalization with  cells expressing tyrosine hydroxylase (TH) and neuropeptide Y (NPY) in the cranial cervical ganglion (CCG) and celiac  plexus in rats.

  Material and methods

  Material and methods. We used 30 white male Wistar rats of six age groups (5 rats per group): newborn pups, 10-,  20-, 30-, and 60-day-old pups, and 24-month-old pups. We incubated their ganglia sections with primary antibodies  against SST, NPY, and TH, as well as with secondary antibodies conjugated with fluorochromes. We evaluated the  ratio between immunoreactive (IR) neurons with a visible nucleolus and excessive fluorescence and the total number  of neurons, as well as the average cross-sectional area, by ImageJ software (NIH, USA).

Results

Results. SST-IR neurons were not found in the CCG. However, the immunoreaction (as granules) was revealed in  most perikaryons at the celiac plexus for SST and NPY with a rather homogeneous distribution for TH. The ratio of  ST-IR neurons reached 33% in pups, doubled during the first month of life, and then remained constant (70–73%).  No statistically significant differences were found between the ratios of SST-IR neurons of the cranial mesenteric  ganglion (CMG) and celiac ganglion (CG) for all age groups. From the moment of birth to 60 days of life, the  average cross-sectional area of SST-IR neurons in the CG and CMG increased by 3.4–3.9 times and then did not  change until 24 months. From the 20th day of life, the average cross-sectional area of SST-IR neurons in the CG  was significantly higher than that in the CMG. All SST-IR neurons in all age groups expressed TH, while 90–94% of  neurons expressed NPY.

 Conclusions

 Conclusions. The content of ST-IR neurons in different sympathetic nodes is not the same: they are absent in the  CCG, and their ratio and area in the celiac plexus increase during early postnatal development. This may be due to  the peculiarities of innervated target organs.  

 Цель

 Цель. Определить содержание нейронов, экспрессирующих соматостатин (SST), и его колокализацию с тирозингидроксилазой (TH) и нейропептидом Y (NPY) в краниальном шейном ганглии (КШГ) и солнечном сплетении крысы.

Материалы и методы

Материалы и методы. Работа выполнена на 30 белых крысах-самцах линии Wistar шести возрастных групп (по 5 крыс в каждой): новорожденные, 10-, 20-, 30-, 60-суточные, 24-месячные. Срезы ганглиев инкубировали с первичными антителами к SST, NPY, TH и вторичными, конъюгированными с флуорохромами. Определяли долю иммунореактивных (IR) нейронов с видимым ядрышком и с флуоресценцией, превышающей фоновое свечение, по отношению к общему числу нейронов, а также среднюю площадь сечения с помощью программы Image J (NIH, США).

Результаты

Результаты. SST-IR нейроны отсутствовали в КШГ. Иммунореактивный материал обнаруживался в области тел большинства нейронов солнечного сплетения в виде гранул для SST и NPY и располагался относительно гомогенно для TH. Доля SST-IR нейронов составляла 33% у новорожденных, увеличивалась в два раза в первый месяц жизни и далее оставалась постоянной (70–73%). Не установлено статистически значимых различий по долям SST-IR нейронов между краниальным брыжеечным ганглием (КБГ) и чревным ганглием (ЧГ) во всех возрастных группах. С момента рождения и до 60 суток жизни средняя площадь сечения SST-IR нейронов в ЧГ и КБГ увеличивалась в 3,4–3,9 раза и далее не менялась до 24 мес. С 20-х суток жизни средняя площадь сечения SST-IR нейронов в ЧГ была статистически значимо выше, чем в КБГ. Все SST-IR нейроны во всех возрастных группах содержали TH и 90–94% нейронов колокализовали NPY.

Заключение

Заключение. Содержание SST-IR нейронов в различных симпатических узлах гетерохронно: они отсутствуют в КШГ, а их доля и площадь в солнечном сплетении в раннем постнатальном онтогенезе увеличивается. Это может быть связано с особенностями иннервируемых органов-мишеней

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The authors declare that there are no conflicts of interest present.