Preview

Sechenov Medical Journal

Advanced search

REPROGRAMMING OF SPLENIC MONONUCLEAR PHAGOCYTE PHENOTYPE DURING MODELING OF SYSTEMIC JUVENILE IDIOPATHIC ARTHRITISIN WISTAR RATS

Full Text:

Abstract

Purpose. This study is devoted to the evaluation of the number of splenic alternatively activated (M2, CD163+) macrophages and other mononuclear phagocytes after arthritis development in a model of systemic juvenile arthritis in Wistar rats (with and without modification by doxycycline or dexamethasone). Methods. Cell phenotypes were assessed by flow cytometric analysis. Percentages of classically-activated phagocytes were estimated (M1, CD163-). Results. The indexes of dominating phenotypes are assessed for spleen (no significant differences in animal groups). In comparison we demonstrate indexes for the blood mononuclear phagocytes (significant changes were observed). Nevetherless, we have demonstrated phenotypical changes of splenic phagocyte population at whole. Conclusions. Redistribution of the tissue alternatively-activated cells of phagocyte population in spleen was found to be significantly different in animal groups with doxycycline and dexamethasone administration.

About the Author

В. Сахаров
ФГАОУ ВО Первый МГМУ им. И.М. Сеченова Минздрава России (Сеченовский Университет)
Russian Federation


References

1. Litvitskiy P, Sakharov V, Alexeeva E, et al. U0212 Model of Arthritis with The Typical Systemic Manifestations in Wistar Rats. Annals of the Rheumatic Diseases. 2016; 75:264

2. Литвицкий П.Ф., Сахаров В.Н., Алексеева Е.И., Маянский Н.А. Способ создания биологической модели системного ювенильного идиопатического артрита в эксперименте. Патент 2 612 843 C1. Заявка от 18.02.2016. Дата регистрации 13.03.2017

3. R Core Team (2016). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/

4. Krzywinski M., Altman N. Points of significance: Visualizing samples with box plots. Nat Methods. 2014; 11(2): 119- 20 http://www.nature.com/nmeth/journal/v11/n2/full/ nmeth.2813.html (дата обращения: 26.05.2016)

5. Сахаров В.Н., Литвицкий П.Ф. Алгоритм оценки процесса активации макрофагов с целью определения прогноза течения и эффективности лечения заболеваний человека. Вестник РАМН. 2015; 70(4): 493-498

6. Litvitskiy P., Sakharov V., Alexeeva E., Mayansky N., Zakirov R. 0105 Doxycycline and dexamethasone induced reprogramming of peripheral blood mononuclear cells in a model of arthritis with the systemic manifestations in Wistar rats. Annals of the Rheumatic Diseases. 2017; 76(Suppl.2):1082-1083 doi: 10.1136/annrheumdis-2017-eular.3996


Review

Views: 261


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 2218-7332 (Print)
ISSN 2658-3348 (Online)