Development and validation of the methods for determination of tenophovir in the blood plasma of rabbits
Abstract
Scribes development and validation of HPLC method with UV detection at 260 nm for determination of tenofovir in rabbit plasma. Plasma samples were treated by protein precipitation using methanol. HPLC analysis was performed on Waters Atlantis T3 column (5 μm, 4,6 x 150 mm), with precolumn Waters Atlantis T3 (5 μm, 4,6 x 20 mm). Acetonitrile –phosphate buffer pH 5.70 (16:84) at 1.0 mL/min was used as mobile phase. The standard curve was linear over the range 0.23 μg/ml to 112,98 μg/ml of tenofovir in plasma. Method precision (RSD, %) was 6.08 % to 12.36 % determined on spiked samples. The accuracy of the method (ε, %) was – 2.96 % to 14.55 %. The lower limit of quantification was 0.23 μg/ml. The method was applied to preclinical pharmacokinetics study of tenofovir drug products in rabbits.
About the Authors
Yu. V. Medvedev
First MSMU named after I. M. Sechenov
Russian Federation
Russian Federation
assistant of the chair of pharmaceutical chemistry with a course of toxicological chemistry
G. V. Ramenskaya
First MSMU named after I. M. Sechenov
Russian Federation
Russian Federation
Doctor of pharmacy, prof., head of the chair of pharmaceutical chemistry with a course of toxicological chemistry
I. E. Shokhin
First MSMU named after I. M. Sechenov
Russian Federation
Russian Federation
PhD, assistant of the chair of pharmaceutical chemistry with a course of toxicological chemistry
T. A. Yarushok
First MSMU named after I. M. Sechenov
Russian Federation
Russian Federation
post-graduate student of the chair of pharmaceutical chemistry with a course of toxicological chemistry
M. A. Sheveleva
First MSMU named after I. M. Sechenov
Russian Federation
Russian Federation
PhD, assistant of the chair of pharmaceutical chemistry with a course of toxicological chemistry
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