The determination of topiramate in plasma rabbit blood by HPLC with specific derivatization
Abstract
Тhe paper of Shohin I.E., Ramenskaya G.V., Medvedev Yu.V., Yarushok T.A., Sheveleva M.A.1 is devoted to development and validation of HPLC method determination of topiramate in rabbit plasma with precolumn derivatization. Topiramate was extracted from plasma by dichloromethane. Derivatization was performed by dansyl chloride in borate buffer. HPLC analysis was performed on C18 column (3 μm, 4,6 x 75 mm). Acetonitrile – 25 mM phosphate buffer pH 2.50 (30:70) at 1.0 mL/min was used as mobile phase. The standard curve was linear over the range 0.95 μg/ml to 47.32 μg/ml of topiramate in plasma. Within-run precision was 1.55 % to 2.12 % and between-run precision was 1.67 % to 8.09 % determined on spiked samples. The accuracy of the method was 1.14 % to 19.62 % (within-run) and 1.74 % to 19.25 (between-run). The lower limit of quantification was 0.95 μg/ ml. The method was applied to preclinical pharmacokinetics study of topiramate drug products in rabbits.
About the Authors
G. V. Ramenskaya
First MSMU named after I. M. Sechenov
Russian Federation
Russian Federation
Doctor of pharmacy, prof., head of the chair of pharmaceutical chemistry with a course of toxicological chemistry
M. A. Sheveleva
First MSMU named after I. M. Sechenov
Russian Federation
Russian Federation
PhD, assistant of the chair of pharmaceutical chemistry with a course of toxicological chemistry
I. E. Shokhin
First MSMU named after I. M. Sechenov
Russian Federation
Russian Federation
PhD, assistant of the chair of pharmaceutical chemistry with a course of toxicological chemistry
Yu. V. Medvedev
First MSMU named after I. M. Sechenov
Russian Federation
Russian Federation
assistant of the chair of pharmaceutical chemistry with a course of toxicological chemistry
T. A. Yarushok
First MSMU named after I. M. Sechenov
Russian Federation
Russian Federation
post-graduate student of the chair of pharmaceutical chemistry with a course of toxicological chemistry
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