Preview

Sechenov Medical Journal

Advanced search

Activity of renal organic anion transporters in a model of ischemia and reperfusion injury in vitro

https://doi.org/10.47093/22187332.2018.4.25-27

Full Text:

Abstract

The aim of the work is to study the functional characteristics of SLC transporters of organic anions: OAT1 and OAT3 in normal conditions and in model ischemia/reperfusion injury.Materials and methods. The HEK293 cell line was used as a model for the study. Conditions of ischemia/reperfusion injury were created by the previously described method. The activity of the transporters was assessed by the capture of the marker substrate - fluorescein. The concentration of fluorescein was measured using a plate fluorimeter. The results were normalized by the amount of total protein.Results. In condition of ischemia/reperfusion injury, the activity of organic anion transporters decreased in comparison with the norm. The data obtained allow us to conclude that in conditions of ischemia/reperfusion injury, the concentration of dicarboxylic acids in the cell is low, which in turn can lead to a decrease activity of transporters.

About the Authors

V. A. Evteev
Scientific Centre for Expert Evaluation of Medicinal Products
Russian Federation


R. E. Kazakov
Scientific Centre for Expert Evaluation of Medicinal Products
Russian Federation


A. B. Prokof'ev
Scientific Centre for Expert Evaluation of Medicinal Products
Russian Federation


I. A. Mazerkina
Scientific Centre for Expert Evaluation of Medicinal Products
Russian Federation


N. D. Bunyatyan
Scientific Centre for Expert Evaluation of Medicinal Products
Russian Federation


References

1. Nigam S.K, Bush K.T, Martovetsky G et al. The Organic Anion Transporter (OAT) Family: A Systems Biology Perspective. Physiol Rev 2015; 95 (1): 83-123.

2. Forrest .LR, Kramer R, Ziegler C. Thestructural basis of secondary active transport mechanisms. Biochim Biophys ActaBioenerg 2011; 1807 (2): 167-88.

3. Hagos Y, Natascha A. Wolff. Assessment of the Role of Renal Organic Anion Transporters in Drug-Induced Nephrotoxicity. Toxins 2010; 2: 2055-82.

4. Cihlar T, Ho E.S. Fluorescence - based assay for the interaction of small molecules with the human renal organic anion transporter 1. Anal Biochem 2000; 283 (1): 49-55.


Views: 185


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 2218-7332 (Print)
ISSN 2658-3348 (Online)