VEGF-C（血管内皮生长因子C） 在腹内高压模拟实验中的肾脏损伤生物标记物

摘要
淋巴管新生在肾损伤时肾实质炎症过程的发展中扮演着重要的角色。血管内皮生长因子C（VEGF-C）是调节淋巴管新生的细胞因子，因而是具有潜力的急性肾损伤的早期生物标记物。
目的
利用新生鼠模拟实验，研究在严重程度和持续时间不同的腹内高压（IAH）背景下VEGF-C在肾脏匀浆和血清中的浓度，并确定其与肾实质形态学变化的相互关系。
材料和方法
实验在50只新生实验白鼠进行。实验鼠分为5组，每组10只：第1组和第2组伴有轻度的腹内高压，持续时间分别为5天和10天。第3组和第4组有严重的腹内高压，持续时间分别为5天和10天。第5组为对照组。通过在膀胱测压法的控制下将无菌凡士林注射入腹腔至预定水平的腹内高压来对其进行模拟。VEGF-C含量通过酵素结合免疫吸附分析法(ELISA，Enzyme-linked immunosorbent assay） 测量。使用徕卡DM2000显微镜对活检材料进行形态学研究并照相。使用曼-惠特尼U检验Mann-Whitney，克鲁斯卡尔-沃利斯检验（Kruskal-Wallis），威尔科克森符号秩检验Wilcoxon以及单向方差分析ANOVA来进行统计分析。
结果
所有组的肾脏匀浆中的VEGF-C水平均升高（pc < 0.001）； VEGF-C增加的程度取决于腹内高压的严重程度（p < 0,05），而不取决于腹内高压的持续时间。VEGF-C血清水平仅在第3组增加（pc = 0.011）。形态学上观察到水肿性变性：肾小管上皮的高度改变，间质性水肿增加重，肾小球的尿路扩张。肾实质形态变化的程度取决于腹内高压的严重程度和持续时间。
结论
在严重程度及持续时间各异的腹内高压背景下，实验白鼠的肾实质形态学变化与肾脏匀浆中的VEGF-C水平变化存在关联性。

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