Preview

Sechenov Medical Journal

Advanced search

DYNAMICS OF EXPRESSION OF CALBINDIN AND CALRETINININ THE NEURONS OF THE INTESTINE IN POSTNATAL ONTOGENESIS

Abstract

The work was done on female Wistar rats aged 1, 10, 20, 30, 60 days, 1 and 2 years after birth (5 animals in each age group). Identification of neurons containing calbindin and calretinin, was performed using labeled antibodies. Secondary antibodies were conjugated with FITC and CY3 fluorochrome. Analysis of drugs was carried out on a fluorescent microscope with the appropriate filter set and a cooled digital CCD camera Tucsen TCC 6.1ICE. To study the size and percentage of neurons in digital images of histological preparations the program Image J was used. In the early postnatal ontogenesis there is an increase in the share of calbindin- and calretinin - containing neurons in the intramural nodes of the intestine, which is the opposite of agerelated changes in the content of calciumbinding proteins in the cerebrospinal and the sympathetic nodes

About the Author

A. F. Budnik
Federal State Budgetary Educational Institution of Higher Education «Kabardino-Balkarian State University named after H.M. Berbekov»
Russian Federation


References

1. Kreutz M.R., Naranjo J.R., Koch K.W., Schwaller B. The neuronal functions of EF-hand Ca2+-binding proteins. Front. Mol. Neurosci. 2012; 11(5): 92.

2. Schwaller B. Calretinin: from a “simple” Ca2+ buffer to a multifunctional protein implicated in many biological processes. Front. Neuroanat. 2014; 5(8): 3.

3. Викулов А.Д., Маргазин В.А., Бойков В.Л., Каунина Д.В. Кальций - важнейший регулирующий фактор жизнедеятельности организма спортсменов-пловцов. Спортивная медицина: наука и практика. 2016; 2: 10-15.

4. Andressen C., Blumcke I., Celio M.R. Calcium-binding proteins: selective markers of nerve cells. Cell Tissue Res. 1993; 271: 181-208.

5. Маслюков П.М., Коробкин А.А., Коновалов В.В., Порсева В.В. и др. Возрастное развитие кальбиндин-иммунопозитивных нейронов симпатических узлов крысы. Морфология. 2012; 141(1): 77-80.

6. Masliukov P.M., Korobkin A.A., Nozdrachev A.D., Timmermans J.P. Calbindin-D28k immunoreactivity in sympathetic ganglionic neurons during development. Auton. Neurosci. 2012; 167(1-2): 27-33.

7. Sayegh A.I., Ritter R.C. Morphology and distribution of nitric oxide synthase-, neurokinin-1 receptor-, calretinin-, calbindin-, and neurofilament-M-immunoreactive neurons in the myenteric and submucosal plexuses of the rat small intestine. Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 2003; 271(1): 209-216.

8. Maslyukov P.M., Nozdrachev A.D., Timmermans J.-P. Agerelated characteristics of the neurotransmitter composition of neurons in the stellate ganglion. Neurosci. Behav. Physiol. 2007; 37(4): 349-353.

9. Чучков В.М., Николенко В.Н., Полякова О.Л., Кашин М.И. Гистоархитектоника и морфологические особенности адренергических нервных терминалей в тканях постоянного зуба у детей. Здоровье, демография, экология финно-угорских народов. 2015; 3: 68-69.

10. Каркищенко В.Н., Каркищенко Н.Н. Методы доклинических исследований в спортивной фармакологии. Спортивная медицина: наука и практика. 2013; 1: 7-17.

11. Yano S., Tokumitsu H., Soderling T.R. Calcium promotes cell survival through CaM-K kinase activation of the protein-kinase-B pathway. Nature. 1998; 396: 584-587.


Review

Views: 499


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 2218-7332 (Print)
ISSN 2658-3348 (Online)