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Creatine phosphate preconditioning reduces ischemiareperfusion injury in isolated rat heart


Aim. To study the effect of simultaneous administration of creatine phosphate immediately before ischemia on cardiodynamic parameters and biomarkers of oxidative stress in the coronary venous blood flow during retrograde perfusion in an isolated rat heart.
Materials and methods. 20 Wistar albino rats were divided into 2 groups: group 1 (control) and group 2 (experimental), 10 rats per group. Cannulation and retrograde perfusion of aorta of an isolated rat heart with Krebs–Henseleit buffered solution by Landendorff was performed. Both groups underwent ischemia-reperfusion injury, which included global ischemia for 20 minutes followed by reperfusion for 30 minutes. The group 2 (experimental) was preconditioned with creatine phosphate at a dose of 0.2 mmol/l for 5 min before ischemia. We registered cardiodynamic parameters and indicators of oxidative stress at the point of stabilization, at the 1st and 30th minutes of reperfusion.
Results. With the impact of creatine phosphate at the 30th minute of reperfusion in the group 2 in comparison with group 1, there was found an increase in the maximum and minimum speed of pressure elevation in the left ventricle (1.7 and 1.9 times, respectively), and of systolic and diastolic pressure in the left ventricle (1.5 and 1.6 times, respectively). Biomarkers of oxidative stress (lipid peroxidation index, nitrites, superoxide anion radical and hydrogen peroxide) were also statistically significantly lower in the group 2 after the 1st minute of reperfusion (by 1.2 times, by 1.4 times, by 2.8 times and 1.9 times, respectively), and after the 30th minute (1.3 times, 2.1 times, 1.9 times and 2.1 times, respectively).
Conclusion. The administration of creatine phosphate into the coronary flow 5 minutes before the onset of ischemia has a protective effect on myocardial contractility. Reduction of oxidative stress and damage can be considered as a protective effect of creatine phosphate.

About the Authors

L. A. Gadjieva
Medical and Sanitary Unit of the Ministry of Internal Affairs of Russia in the Republic of Dagestan
Russian Federation

Louisa A. Gadjieva, applicant for Cand. of Sci. (Medicine), internist, urgent medical aid department

29а–31, Pushkina str., Makhachkala, 367000

S. B. Bolevich
Sechenov First Moscow State Medical University (Sechenov University)
Russian Federation

Sergey B. Bolevich, Dr. of Sci. (Medicine), Professor, Head of the Human Pathology Department

8/2, Trubetskaya str., Moscow, 119991

V. Jakovlevich
Sechenov First Moscow State Medical University (Sechenov University); University of Kragujevac
Russian Federation

Vladimir Jakovljevic, MD, PhD, Professor, Dean of the Faculty of Medical Sciences

8/2, Trubetskaya str., Moscow, 119991

69, Svetozar Markovic str., Kragujevac, 34000

I. A. Omarov
Health-Related Center of Ministry of External Affairs of Russian Federation
Russian Federation

Israpil A. Omarov, Cand. of Sci. (Medicine), orthopedic dentist, Medical and health center

2, bld. 2, Smolenskaja quay, Moscow, 121099

H. A. Ordashev
Dagestan State Medical Univesity

Hasan A. Ordashev, Cand. of Sci. (Medicine), Associate Professor, Head of the Department of Surgical Dentistry and Maxillofacial Surgery with advanced training for doctors

1, Lenin Square, Makhachkala, 367000

M. K. Kartashova
Sechenov First Moscow State Medical University (Sechenov University)
Russian Federation

Maria K. Kartashova, 6th year student of Medical Faculty

8/2, Trubetskaya str., Moscow, 119991


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