Results of application for the treatment of bioingineering construction for occlusive vascular diseases in animal experiments

Objective: study of the application in experimental use of bioengineering construction for the treatment of occlusive vascular diseases. Materials and methods: bioengineering construction is a stent, in which the stiffeners further provided with at least one fixed reservoir therein living culture cells and culture medium. Bioengineering structure is a stent, in which the stiffeners further provided with at least one fixed reservoir therein living culture cells and culture medium. A stent implantation in experimental animals (9 mongrel dogs) was performed under intravenous anesthesia. In seven experiments, 70 days after the operation was carried out repeated selective angiography to assess the patency of stents coated with cultured fibroblasts. In two experiments 2 years after implantation bioengineering construction with rotary aortography with 3D-reconstruction of the renal arteries is also passable, adequate positioning of the stent, evidence of thrombosis of the distal bed is not revealed. Disaggregation treatment in these two animals for two years and was not performed. All experimental animals underwent angiography and were euthanized. The aortal parts with the renal arteries and kidneys were got after autopsy. The stented parts of renal arteries were endothelializied even layer. On a section, we could clearly see location of the stent cells relative to the vessel wall thickness. Сonclusion: Stents coated with cultured fibroblasts, have several properties that provide them advantages over the known products, and in particular: good fi x biomaterial; initially not thrombogenic; stimulate rapid and uniform endothelialization of the stented portion of the vessel; do not lead to restenosis, as do not provoke inflammatory reactions at the implantation site.

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